Rpe Qiagen: Buffer

The primary role of Buffer RPE is to remove traces of salts and other contaminants that remain on the silica membrane after initial lysis and primary washing steps (such as those using Buffer RW1 or Buffer RWT).

While the exact proprietary formulation is held by Qiagen, Buffer RPE is broadly understood to contain Ethanol (or Isopropanol in some specific RNA variants) and a low concentration of salts in a Tris-HCl buffered solution. buffer rpe qiagen

The purification process involves three distinct phases where Buffer RPE acts in the second phase (Washing): The primary role of Buffer RPE is to