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| Strengths | Limitations | |-----------|--------------| | • Integration of provides a robust mechanistic narrative. • Use of CRISPR lines allows causal inference about sperm traits. • Cross‑taxonomic validation (flies + beetles) increases generality. | • Microfluidic chambers, while sophisticated, may not capture all biochemical cues present in vivo (e.g., seminal fluid proteins). • Focus on Drosophila limits direct extrapolation to mammals where sperm cooperation (e.g., bundles) is common. • The ABM assumes constant female behavior; dynamic female choice could alter outcomes. | saki kawanami spermmania